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Kevin Wang Laboratory

Lab Location/ Complete Address:

D-114, Science and Health Profession Building

Department of Natural Sciences

Northeastern State University at Broken Arrow

3100 East New Orleans Street

Broken Arrow, OK 74014

 

Lab Lead Contact:

Dr. Kevin Yueju Wang

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Lab Personnel:

PI: Dr. Kevin Yueju Wang

Research Scientist: Dr. Frank Yau

 

Research Description:

My research interests focus on plant biotechnology and the use of transgenic plants for medicinal purposes.

Develop novel transgenic plants for production of recombinant clot-busting pharmaceutical proteins for acute ischemic stroke patient without increasing risk of brain bleeding (hemorrhage) and neurons damage. Every year, more than 750,000 people suffer from stroke with 150,000 leading to deaths in the United States. About 550,000 are first strokes, and 85% of which are ischemic. It is the 3rd leading cause of death in the U.S. Stroke is the major cause of serious, long-term disability with more than 4 million stroke survivors in the United States. Recently, vampire bat (Desmodus rotundus) salivary plasminogen activators (DSPAs) have been found more active than the t-PA without increasing side effects. We hypothesize that the transgenic plant system can offer the potential for rapid and economical scale-up DSPAs.

Even approaches for introduction transgenes into plants become routine, the random insertion of transgene into the plant genome generates unpredictable outcomes for the transgene, multiple copies, varies gene expression and the screen for the good lines are labor intensive and time consuming. Final product carrying antibiotic resistance genes (selectable marker) raises transgenic plant safety concern. Those defects of the current plant transformation methods can hinder the future plant biotechnology application needs. Many studies have focused on integration of multiple genes in a single transgenic plant with selectable marker gene removal. Among those tools, application of site-specific recombination systems has demonstrated a promising technology to effectively target the foreign genes into a known site with unnecessary gene removal. Here, we use of the site-specific recombination systems to produce the maker-free transgenic plants with single copy transgene insertion.

 

Instrument and Technique Specialties:

Faculty Resource Available: Two Refrigerator/freezers, small flammablematerials storage freezer, ultra low freezer, incubators, high speed microfuge, low speed centrifuge, constant temperature air shaker, electroporator, thermomixer, water baths, pH meter, balances, water purification unit, sterilizer, sonicator, electrophoresis equipment, hybridization equipment, gel documentation system, spectrophotometer and PCR machine

Major Equipment Available for Plant project:

Three laminar flow hoods (ESCO) for plant cell and tissue culture work.

One plant growth chamber (Thermo) for seed germination and development.

Vacuum infiltration system for both transient and stable plant transformation.

Real-time PCR machine (BioRad) combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection to analyze RNA expression.

Profinia Protein Purification Instrument (Biorad) replaces traditional gravity-flow techniques with a complete system for the purification and desalting of proteins. It is designed for high-throughout protein purification.

 

Funding Sources:

NIH/INBRE, Faculty Development Research

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Our Mission:

To develop bioscience research in Tulsa and to position Tulsa to be a leader in bioscience education, training, research and innovation by utilizing the assets of all area institutions of higher education.
-TABERC

 

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