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Sapna Das Bradoo Laboratory

 

Lab Location/ Complete Address:

133, Science and Health Profession Building

Department of Natural Sciences

Northeastern State University at Broken Arrow

3100 East New Orleans Street

Broken Arrow, OK 74014

 

Lab Lead Contact:

Dr. Sapna Das Bradoo

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Lab Personnel:

PI: Dr. Sapna Das Bradoo

Lab technician: Ms. Brandy Fultz

Graduate student: Mr. Casey Eddington

Graduate student: Mr. Chance Hendrix

Undergraduate student: Mr. Mohamed Abdelmonem

Undergraduate student: Mr. Shane Benjamin

 

Research Description:

The broad objective of my research is to study proteins involved in DNA replication. DNA replication is an important step in the eukaryotic cell cycle, and accurate duplication of the genome is essential for cell viability. Failure to do so results in DNA breakage during replication and increased genome instability, which can cause cancer (Das-Bradoo and Bielinsky, 2010). It is crucial, therefore, to understand the entire process of DNA replication.

My research will employ biochemical, molecular biology techniques, and genetics in the model organism, Saccharomyces cerevisiae. The roles of specific DNA replication proteins will be studied, which will help to gain a better understanding of their function.  

Our main focus is on an essential DNA replication protein, known as minichromosome maintenance protein 10 (Mcm10). The evolutionary conserved Mcm10 has been implicated in both the initiation and elongation steps of DNA replication. S. cerevisiae Mcm10 is cell cycle regulated and interacts with the catalytic subunit of polymerase alpha, the only enzyme capable of starting DNA replication in vivo. Furthermore, I have shown that Mcm10 is di-ubiquitinated and in its di-ubiquitinated form Mcm10 releases polymerase alpha and interacts with proliferating cell nuclear antigen (PCNA) (Das-Bradoo et al., 2006). PCNA is the processivity factor for polymerase delta and polymerase epsilon, the polymerases that carry out the bulk DNA synthesis after polymerase alpha has started the process. Therefore, Mcm10 appears to be a key coordinator of the switch between polymerase alpha and polymerase delta/epsilon and is a putative target for anti-cancer therapy.

Students in my lab will carry out experiments targeted to achieve a better understanding of Mcm10 and its functional significance.

 

Funding Sources:

NIH/INBRE, Faculty Development Research

Our Contact Details


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Our Mission:

To develop bioscience research in Tulsa and to position Tulsa to be a leader in bioscience education, training, research and innovation by utilizing the assets of all area institutions of higher education.
-TABERC

 

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